2019-02-19 18:13:272025-05-11 11:10:16
locus
BSU25330
BSU_25330
outlinks
bsu
BSU25330
BSU_25330
The protein
Catalyzed reaction/ biological activity
hydrolysis of c-di-AMP to 5'-pApA [Pubmed|25583510]
hydrolysis of c-di-AMP to 5'-pApA [Pubmed|25583510]
3',3'-c-di-AMP + H2O --> 5'-O-phosphonoadenylyl-(3'→5')-adenosine + H+ (according to UniProt)
The protein
[SW|Domains]
[SW|HD domain] (aa 497 ... 663) (according to the Interpro database)
[SW|HD domain] (aa 501-643) (according to UniProt)
Biological materials
Mutant
MGNA-C432 (yqfF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2430 NBRP B. subtilis, Japan]
SM-GN1 (''pgpH-spc''), available in [SW|Anne Galinier]'s and [SW|Boris Grke]'s labs [pubmed|16272399]
BKE25330 (''[[gene|pgpH]]''::''erm'' without terminator, available in the BGSC and in [SW|Jrg Stlke]'s lab) [pubmed|28189581]
GP2033 (''[[gene|pgpH]]''::''tet'', available in [SW|Jrg Stlke]'s lab)
GP2034 (''[[gene|pgpH]]''::''erm'' without terminator, available in [SW|Jrg Stlke]'s lab) [Pubmed|26240071]
GP2040 (''[[gene|gdpP]]''::''spc'' ''[[gene|pgpH]]''::''erm'' without terminator, available in [SW|Jrg Stlke]'s lab) [Pubmed|26240071]
GP2049 (''[[gene|pgpH]]''::''cat'' without terminator, available in [SW|Jrg Stlke]'s lab)
BKE25330 ([[gene|pgpH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAGAACCTCCTCTTGAA, downstream forward: _UP4_GAGGCTACTAAGAAGGTGAA
BKK25330 ([[gene|pgpH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAGAACCTCCTCTTGAA, downstream forward: _UP4_GAGGCTACTAAGAAGGTGAA
MGNA-C432 (yqfF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2430 NBRP B. subtilis, Japan]
SM-GN1 (''pgpH-spc''), available in [SW|Anne Galinier]'s and [SW|Boris Görke]'s labs [pubmed|16272399]
BKE25330 (''[[gene|pgpH]]''::''erm'' without terminator, available in the BGSC and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
GP2033 (''[[gene|pgpH]]''::''tet'', available in [SW|Jörg Stülke]'s lab)
GP2034 (''[[gene|pgpH]]''::''erm'' without terminator, available in [SW|Jörg Stülke]'s lab) [Pubmed|26240071]
GP2040 (''[[gene|gdpP]]''::''spc'' ''[[gene|pgpH]]''::''erm'' without terminator, available in [SW|Jörg Stülke]'s lab) [Pubmed|26240071]
GP2049 (''[[gene|pgpH]]''::''cat'' without terminator, available in [SW|Jörg Stülke]'s lab)
BKE25330 ([[gene|pgpH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAGAACCTCCTCTTGAA, downstream forward: _UP4_GAGGCTACTAAGAAGGTGAA
BKK25330 ([[gene|pgpH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAGAACCTCCTCTTGAA, downstream forward: _UP4_GAGGCTACTAAGAAGGTGAA
Biological materials
lacZ fusion
pGP190 (in [[protein|pAC7]]), available in [SW|Stlke] lab
pGP190 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab
References
Reviews
The protein
Protein family
PgpH phosphodiesterase family (single member, according to UniProt)
labs
[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]